Lassar A B, Martin P L, Roeder R G
Science. 1983 Nov 18;222(4625):740-8. doi: 10.1126/science.6356356.
Class III genes require multiple cellular factors for transcription by RNA polymerase III; these genes form stable transcription complexes, which in the case of Xenopus 5S genes are correlated with differential expression in vivo. The minimal number and identity of the factors required to form both stable and metastable complexes on three class III genes (encoding, respectively, 5S RNA, transfer RNA, and adenovirus VA RNA species) were determined. Stable complex formation requires one common factor, whose recognition site was analyzed, and either no additional factors (the VA gene), a second common factor (the transfer RNA gene), or a third gene-specific factor (the 5S gene). The mechanism of stable complex formation and its relevance to transcriptional regulation were examined in light of the various factors and the promoter sequences recognized by these factors.
III类基因需要多种细胞因子才能由RNA聚合酶III进行转录；这些基因形成稳定的转录复合物，在非洲爪蟾5S基因的情况下，其与体内的差异表达相关。确定了在三个III类基因（分别编码5S RNA、转运RNA和腺病毒VA RNA种类）上形成稳定和亚稳定复合物所需的因子的最少数量和特性。稳定复合物的形成需要一种共同因子，对其识别位点进行了分析，此外还需要要么不需要其他因子（VA基因）、要么需要第二种共同因子（转运RNA基因）、要么需要第三种基因特异性因子（5S基因）。根据各种因子以及这些因子识别的启动子序列，研究了稳定复合物形成的机制及其与转录调控的相关性。
