胸腺嘧啶前含有脲脱氧核糖残基的寡核苷酸的溶液构象。
Solution conformation of an oligonucleotide containing a urea deoxyribose residue in front of a thymine.
作者信息
Gervais V, Guy A, Téoule R, Fazakerley G V
机构信息
Service de Biochimie et de Génétique Moléculaire, DBCM, Centre d'Etudes de Saclay, Gif-sur-Yvette, France.
出版信息
Nucleic Acids Res. 1992 Dec 25;20(24):6455-60. doi: 10.1093/nar/20.24.6455.
Abstract
Urea residues are produced by ionizing radiation on thymine residues in DNA. We have studied an oligodeoxynucleotide containing a thymine opposite the urea residue, by one and two dimensional NMR spectroscopy. The urea deoxyribose exists as two isomers with respect to the orientation about the peptide bond. For the trans isomer we find that the thymine and urea site are positioned within the helix and are probably hydrogen bonded. The oligonucleotide adopts a globally B form structure although conformational changes are observed around the mismatch site. A minor species is observed, in which the urea deoxyribose and the opposite base adopt an extrahelical position and this corresponds to the isomer cis for the peptide bond.
摘要
尿素残基是由DNA中胸腺嘧啶残基的电离辐射产生的。我们通过一维和二维核磁共振光谱研究了一种寡脱氧核苷酸，该寡脱氧核苷酸在与尿素残基相对的位置含有一个胸腺嘧啶。尿素脱氧核糖相对于肽键的取向存在两种异构体。对于反式异构体，我们发现胸腺嘧啶和尿素位点位于螺旋内部，可能形成氢键。尽管在错配位点周围观察到构象变化，但该寡核苷酸整体上呈现B型结构。观察到一种次要物种，其中尿素脱氧核糖和相对的碱基采取螺旋外位置，这对应于肽键的顺式异构体。
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