Nam H W, Kang S W, Choi W Y
Department of Parasitology, Catholic University of Korea, College of Medicine, Seoul, Korea.
Korean J Parasitol. 1998 Dec;36(4):269-75. doi: 10.3347/kjp.1998.36.4.269.
Anti-Neospora caninum antibody was detected in anti-Toxoplasma gondii positive and negative human sera by ELISA, western blot and immunofluorescence assay (IFA). Twelve cases out of 172 (6.7%) Toxoplasma-positive sera cross-reacted with both T. gondii and N. caninum antigens, and one out of 110 Toxoplasma-negative sera reacted with N. caninum antigen by ELISA. By western blot, all 12 sera reacted with T. gondii antigens with various banding patterns but specifically at 30 kDa (SAG1) and 22 kDa (SAG2) bands. With N. caninum antigen, the number of reactive bands was reduced, however a 43 kDa band reacted in three cases in Toxoplasma-positive sera in addition to one in Toxoplasma-negative control sera. All sera of the Toxoplasma-positive group labeled surface membrane of T. gondii, but reacted differently with N. caninum. Fluorescence was detected in surface membrane, subcellular organelles, or both in N. caninum. And one case in the Toxoplasma-negative group also reacted with N. caninum strongly in subcellular organelles. This suggested that the antibody against N. caninum may be present in human sera although the positive rate was very low in this study. The possibility of human infection with N. caninum remains to be evaluated further.
通过酶联免疫吸附测定（ELISA）、蛋白质印迹法和免疫荧光测定（IFA）在抗弓形虫阳性和阴性的人血清中检测抗犬新孢子虫抗体。172份弓形虫阳性血清中有12份（6.7%）与弓形虫和犬新孢子虫抗原均发生交叉反应，110份弓形虫阴性血清中有1份通过ELISA与犬新孢子虫抗原发生反应。通过蛋白质印迹法，所有12份血清均与弓形虫抗原发生反应，呈现出不同的条带模式，但特异性地出现在30 kDa（SAG1）和22 kDa（SAG2）条带处。对于犬新孢子虫抗原，反应条带数量减少，然而除了1份弓形虫阴性对照血清外，弓形虫阳性血清中有3份在43 kDa条带处发生反应。弓形虫阳性组的所有血清均标记了弓形虫的表面膜，但与犬新孢子虫的反应不同。在犬新孢子虫的表面膜、亚细胞器或两者中均检测到荧光。并且弓形虫阴性组中有1例在亚细胞器中也与犬新孢子虫发生强烈反应。这表明尽管本研究中的阳性率非常低，但人血清中可能存在抗犬新孢子虫抗体。人感染犬新孢子虫的可能性仍有待进一步评估。
