大鼠静脉注射[14C]-唾液酸-GM3和[3H]-鞘氨醇-GM1后的动力学及器官分布

Kinetics and organ distribution of [14C]-sialic acid-GM3 and [3H]-sphingosine-GM1 after intravenous injection in rats.

作者信息

Dumontet C, Rebbaa A, Portoukalian J

机构信息

Unité INSERM 218, Centre Léon Bérard, Lyon, France.

出版信息

Biochem Biophys Res Commun. 1992 Dec 30;189(3):1410-6. doi: 10.1016/0006-291x(92)90231-9.

DOI:10.1016/0006-291x(92)90231-9

PMID:1482356

Abstract

Serum kinetics and organ distribution of [14C]-sialic acid-GM3 and [3H]-sphingosine-GM1, administered as an intravenous bolus, were analysed in Wistar rats. [3H]-GM1 and [14C]-GM3 had serum half-lives of 1.4 hours and 1.8 hours, respectively. Three hours after injection 75% of the GM1- and 38% of the GM3-associated label were present in the liver. Smaller yet significant amounts of label were present in the central nervous system, kidneys and lung. In vitro studies showed that [14C]-GM3 and [3H]-GM1 incubated with serum were predominantly bound to the High Density Lipoprotein and the Low Density Lipoprotein fractions. These results suggest a rapid serum clearance of exogenous gangliosides by the liver in rats.

摘要

通过静脉推注给药后，在Wistar大鼠中分析了[14C] - 唾液酸 - GM3和[3H] - 鞘氨醇 - GM1的血清动力学和器官分布。[3H] - GM1和[14C] - GM3的血清半衰期分别为1.4小时和1.8小时。注射后3小时，75%与GM1相关的标记物和38%与GM3相关的标记物存在于肝脏中。在中枢神经系统、肾脏和肺中也存在少量但显著的标记物。体外研究表明，与血清一起孵育的[14C] - GM3和[3H] - GM1主要与高密度脂蛋白和低密度脂蛋白部分结合。这些结果表明大鼠肝脏对外源性神经节苷脂具有快速的血清清除作用。