Venkateswaran K S, Raghuveeran C D, Gopalan N, Agarwal G S, Kaushik M P, Vijayaraghavan R
Defence Research and Development Establishment, Gwalior, India.
Biochem Int. 1992 Dec;28(4):745-50.
Carbamylation of haemoglobin by methyl isocyanate (MIC) was detected by high-performance liquid chromatography (HPLC) using a photodiode array detector following cyclisation of the N-terminal valine into methyl isopropyl hydantoin (MIH). MIH was also synthesised by reaction of MIC with valine, the chromatographic conditions standardised and the spectrum derived by a photodiode array detector recorded for confirmation of the identity of MIH. This HPLC method is specific, sensitive and suitable for the detection of exposure of blood samples to methyl isocyanate.
