Karol M H, Kamat S R
Department of Industrial Environmental Health Sciences, Graduate School of Public Health, University of Pittsburgh, PA 15261.
Bull Eur Physiopathol Respir. 1987 Nov-Dec;23(6):591-7.
Sera from 99 subjects exposed to the industrial gas leak in Bhopal on December 2, 1984 were studied along with sera from guinea pigs exposed to methyl isocyanate (MIC) to determine the production of antibodies specific to (MIC). Each of the four guinea pigs injected with the reactive isocyanate produced MIC-specific antibodies in titres of 1:5120 to 1:10240, when tested with MIC-guinea pig albumin antigen conjugate. Analogous antigens prepared by reaction of MIC with human serum albumin were used to probe production of antibodies in 264 serially obtained human sera from 99 subjects from Bhopal. MIC-specific antibodies belonging to IgG, IgM and IgE classes were detected in eleven subjects. Though titres were low and transient (declining after several months) these findings indicate that the single large exposure to MIC resulted in an immunologic response. This finding was concomitant with chronic respiratory effects following MIC exposure.
对1984年12月2日在博帕尔接触工业气体泄漏的99名受试者的血清以及接触异氰酸甲酯（MIC）的豚鼠血清进行了研究，以确定针对（MIC）的特异性抗体的产生情况。当用MIC-豚鼠白蛋白抗原结合物进行检测时，注射了反应性异氰酸酯的四只豚鼠中的每一只都产生了效价为1:5120至1:10240的MIC特异性抗体。用MIC与人血清白蛋白反应制备的类似抗原用于检测来自博帕尔99名受试者的264份连续采集的人血清中的抗体产生情况。在11名受试者中检测到了属于IgG、IgM和IgE类别的MIC特异性抗体。尽管效价较低且短暂（几个月后下降），但这些发现表明单次大量接触MIC会导致免疫反应。这一发现与接触MIC后的慢性呼吸道效应同时出现。
