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甲基绿可替代偏端霉素A用于形成偏端霉素A/ 4',6-二脒基-2-苯基吲哚C带。

Methyl green is a substitute for distamycin A in the formation of distamycin A/DAPI C-bands.

作者信息

Donlon T A, Magenis R E

出版信息

Hum Genet. 1983;65(2):144-6. doi: 10.1007/BF00286651.

DOI:10.1007/BF00286651
PMID:6654329
Abstract

The DA/DAPI technique has been found to be useful in the identification of specific chromosomal regions on human chromosomes. The realization that distamycin A (DA) is no longer commercially available has necessitated the development of an alternative technique. We describe a technique, MG/DAPI, which substitutes the AT-specific dye methyl green for distamycin A and gives results identical to those of the DA/DAPI technique.

摘要

已发现DA/DAPI技术在鉴定人类染色体上的特定染色体区域方面很有用。由于意识到放线菌素A（DA）已不再有商业供应，因此有必要开发一种替代技术。我们描述了一种技术，即MG/DAPI，它用AT特异性染料甲基绿替代放线菌素A，其结果与DA/DAPI技术相同。

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Hum Genet. 1983;65(2):144-6. doi: 10.1007/BF00286651.
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Methyl green. III. Reaction with desoxyribonucleic acid, stoichiometry, and behavior of the reaction product.甲基绿。III. 与脱氧核糖核酸的反应、化学计量关系及反应产物的性质
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Preferential fluorescent staining of heterochromatic regions in human chromosomes 9, 15, and the Y by D 287/170.D 287/170对人类9号、15号染色体以及Y染色体上异染色质区域的优先荧光染色。
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Comp Cytogenet. 2015 Sep 29;9(4):613-23. doi: 10.3897/CompCytogen.v9i4.5530. eCollection 2015.
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Comp Cytogenet. 2012 Apr 27;6(2):183-97. doi: 10.3897/CompCytogen.v6i2.2950. eCollection 2012.
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Comparative cytogenetic study on two species of the genus Entedon Dalman, 1820 (Hymenoptera, Eulophidae) using DNA-binding fluorochromes and molecular and immunofluorescent markers.利用DNA结合荧光染料以及分子和免疫荧光标记对Entedon属（1820年，达尔曼）的两个物种进行比较细胞遗传学研究（膜翅目，釉小蜂科）
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