Tachibana T, Nawa T
Department of Oral Anatomy, Iwate Medical University School of Dentistry, Morioka, Japan.
Arch Histol Cytol. 1992 Oct;55(4):375-9. doi: 10.1679/aohc.55.375.
The cytochemical localization of Ca(++)-ATPases in Meissner's corpuscles of the palatine mucosa of the Mongolian gerbil was studied. Wachstein-Meisel's medium modified by SALAMA et al. (1987) was used for the demonstration of low-affinity Ca(++)-ATPase or Ca++/Mg(++)-ATPase, whereas the medium devised by Körtje et al. (1990) was used for the demonstration of high-affinity Ca(++)-ATPase. Orthovanadate-containing medium, Ca(++)- or/and Mg(++)-free medium, ATP-free medium and preheated sections were used as controls. The plasma membrane of axon terminals consistently showed a strong reaction for high-affinity Ca(++)-ATPase and a capricious reaction for Ca++/Mg(++)-ATPase. In contrast, plasma membranes of the caveolae of lamellar plates showed a strongly positive reaction for Ca++/Mg(++)-ATPase.
研究了蒙古沙鼠腭黏膜迈斯纳小体中Ca(++)-ATP酶的细胞化学定位。采用SALAMA等人（1987年）改良的Wachstein-Meisel培养基来显示低亲和力Ca(++)-ATP酶或Ca++/Mg(++)-ATP酶，而Körtje等人（1990年）设计的培养基则用于显示高亲和力Ca(++)-ATP酶。含正钒酸盐的培养基、无Ca(++)或/和Mg(++)的培养基、无ATP的培养基以及预热切片用作对照。轴突终末的质膜始终对高亲和力Ca(++)-ATP酶呈现强烈反应，而对Ca++/Mg(++)-ATP酶呈现多变反应。相比之下，板层小窝的质膜对Ca++/Mg(++)-ATP酶呈现强阳性反应。
