Morin P J, Subramanian G S, Gilmore T D
Department of Biology, Boston University, MA 02215.
Biochim Biophys Acta. 1992 Dec 29;1171(2):211-4. doi: 10.1016/0167-4781(92)90124-i.
We have isolated a gene, AAT1, encoding an aspartate aminotransferase (AspAT) from a Saccharomyces cerevisiae genomic library. AAT1 encodes a 451 amino acid protein with a predicted molecular weight of 51,687, which is likely to be the yeast mitochondrial AspAT. Sequence comparison of this yeast AspAT with AspATs from other organisms shows a high degree of homology in regions previously shown to be important for catalysis. However, the yeast mitochondrial AspAT contains four obvious insertions with respect to all other known AspATs, suggesting that the AAT1-encoded protein represents a distinct AspAT.
我们从酿酒酵母基因组文库中分离出了一个编码天冬氨酸转氨酶（AspAT）的基因AAT1。AAT1编码一个含有451个氨基酸的蛋白质，预测分子量为51,687，它可能是酵母线粒体天冬氨酸转氨酶。将这种酵母天冬氨酸转氨酶与来自其他生物体的天冬氨酸转氨酶进行序列比较，发现在先前已证明对催化作用很重要的区域具有高度同源性。然而，相对于所有其他已知的天冬氨酸转氨酶，酵母线粒体天冬氨酸转氨酶含有四个明显的插入片段，这表明由AAT1编码的蛋白质代表一种独特的天冬氨酸转氨酶。
