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乙烯对酿酒酵母代谢的影响。

Effects of ethylene on the metabolism of Saccharomyces cerevisiae.

作者信息

Thomas K C, Spencer M

出版信息

Can J Microbiol. 1978 Mar;24(3):222-7. doi: 10.1139/m78-040.

DOI:10.1139/m78-040
PMID:148318
Abstract

Supply of exogenous ethylene to lactate-grown yeast initially accelerated the rate of ethanol production from glucose, but later reduced the rate, with the overall effect being to reduce the total ethanol production. The rate of ethanol production by ethylene-treated yeast was not changed by removal of metabolic carbon dioxide. However, if CO2 was allowed to build up in the absence of applied ethylene, the ethanol production decreased. Ethylene increased the activities of a number of pentose phosphate and glycolytic pathway enzymes. The largest increase in activity was observed for phosphofructokinase (EC 2.7.1.11), regulatory enzyme of the glycolytic pathway. After an initial stimulation, glucose (and also 3-O-methyl glucose) uptake was reduced by ethylene. Ethylene appears to inhibit non-competitively the glucose transport system.

摘要

向以乳酸为碳源生长的酵母供应外源乙烯,最初会加速葡萄糖产生乙醇的速率,但随后会降低该速率,总体效果是减少乙醇的总产量。经乙烯处理的酵母产生乙醇的速率不会因代谢产生的二氧化碳的去除而改变。然而,如果在不施加乙烯的情况下让二氧化碳积累,乙醇产量会下降。乙烯增加了多种磷酸戊糖途径和糖酵解途径酶的活性。糖酵解途径的调节酶磷酸果糖激酶(EC 2.7.1.11)的活性增加最为显著。在最初的刺激之后,乙烯会降低葡萄糖(以及3 - O - 甲基葡萄糖)的摄取。乙烯似乎以非竞争性方式抑制葡萄糖转运系统。

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