Gancedo J M, Gancedo C
Eur J Biochem. 1979 Nov;101(2):455-60. doi: 10.1111/j.1432-1033.1979.tb19739.x.
Yeast mutants blocked at different steps of the glycolytic pathways have been used to study the inactivation of several gluconeogenic enzymes upon addition of sugars. While phosphorylation of the sugars appears a requisite for the inactivation of fructose 1,6-bisphosphatase and phosphoenol-pyruvate carboxykinase, malate dehydrogenase is inactivated by fructose in mutants lacking hexokinase. The normal inactivation elicited by glucose in a mutant lacking phosphofructokinase indicates that the process does not require metabolism of the sugar beyond hexose monophosphates. A possible role for ATP in the inactivation process is suggested.
已利用在糖酵解途径不同步骤受阻的酵母突变体来研究添加糖类后几种糖异生酶的失活情况。虽然糖类的磷酸化似乎是果糖1,6 -二磷酸酶和磷酸烯醇式丙酮酸羧激酶失活的必要条件，但在缺乏己糖激酶的突变体中，苹果酸脱氢酶会被果糖失活。在缺乏磷酸果糖激酶的突变体中，葡萄糖引发的正常失活表明该过程不需要糖类代谢超过单磷酸己糖。这提示了ATP在失活过程中可能发挥的作用。
