Robertson C R, Pisetsky D S
Division of Rheumatology and Immunology, Durham VA Hospital 27705.
Clin Exp Rheumatol. 1992 Nov-Dec;10(6):589-94.
To evaluate the properties of antibodies to bacterial DNA in the sera of normal human subjects (NHS) and patients with systemic lupus erythematosus (SLE), the effects of ionic strength and pH on their binding to single-stranded DNA (ssDNA) from Micrococcus lysodeikticus (MC) were measured. By ELISA, antibodies to MC ssDNA in NHS showed greater activity at high ionic strength (0.2-1.0 M) than antibodies in lupus sera. Similarly, antibodies in NHS had higher activity at pH 9 than lupus anti-DNA. Competition binding assays indicated, moreover, that NHS anti-DNA showed greater inhibition by DNA than lupus anti-DNA at comparable inhibitor concentrations. Together, these results suggest that antibodies to MC ssDNA in NHS and SLE sera may differ in their mode of interaction with bacterial DNA and that NHS can generate high avidity antibodies to at least certain DNA determinants.
为评估正常人（NHS）和系统性红斑狼疮（SLE）患者血清中抗细菌DNA抗体的特性，测定了离子强度和pH对其与溶壁微球菌（MC）单链DNA（ssDNA）结合的影响。通过酶联免疫吸附测定（ELISA）发现，NHS中抗MC ssDNA抗体在高离子强度（0.2 - 1.0 M）下比狼疮血清中的抗体表现出更高的活性。同样，NHS中的抗体在pH 9时比狼疮抗DNA抗体具有更高的活性。此外，竞争结合试验表明，在相当的抑制剂浓度下，NHS抗DNA比狼疮抗DNA受DNA的抑制作用更强。这些结果共同表明，NHS和SLE血清中抗MC ssDNA抗体与细菌DNA的相互作用模式可能不同，且NHS能够产生针对至少某些DNA决定簇的高亲和力抗体。
