Robertson C R, Gilkeson G S, Ward M M, Pisetsky D S
Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710.
Clin Immunol Immunopathol. 1992 Jan;62(1 Pt 1):25-32. doi: 10.1016/0090-1229(92)90019-k.
Although anti-DNA antibodies are generally considered to be specific markers for systemic lupus erythematosus (SLE), antibodies binding DNA from certain bacterial species can be found in the sera of normal subjects. To characterize the immunochemical properties of these antibodies, the IgG subclass and light chain profile of antibodies to single-stranded micrococcal DNA (MC DNA) in the sera of normal subjects and patients with SLE was determined. The anti-MC DNA response in normal sera was predominantly of the IgG2 subclass with a marked predominance of kappa light chains. In contrast, anti-MC DNA antibodies in SLE sera exhibited all IgG subclasses with a predominance of the IgG1 subclass and both kappa and lambda light chains were represented. These results suggest that antibodies to bacterial DNA in the sera of normal subjects and patients with SLE differ in patterns of immunoglobulin gene expression; the restricted response of normal subjects may be related to the binding to a discrete DNA determinant.
尽管抗DNA抗体通常被认为是系统性红斑狼疮（SLE）的特异性标志物，但在正常受试者血清中也可发现能结合某些细菌种类DNA的抗体。为了表征这些抗体的免疫化学特性，测定了正常受试者和SLE患者血清中抗单链微球菌DNA（MC DNA）抗体的IgG亚类和轻链谱。正常血清中的抗MC DNA反应主要为IgG2亚类，κ轻链明显占优势。相比之下，SLE血清中的抗MC DNA抗体表现出所有IgG亚类，其中IgG1亚类占优势，κ和λ轻链均有表达。这些结果表明，正常受试者和SLE患者血清中针对细菌DNA的抗体在免疫球蛋白基因表达模式上存在差异；正常受试者的受限反应可能与对离散DNA决定簇的结合有关。
