Bunyard M P, Pisetsky D S
Research Service, Durham VA Hospital, N.C. 27705.
Int Arch Allergy Immunol. 1994 Oct;105(2):122-7. doi: 10.1159/000236813.
To assess the human antibody response to bacterial double-stranded (ds) DNA, sera from normal human subjects (NHS) were tested by ELISA for binding to highly purified dsDNA from Micrococcus lysodeikticus (MC). Of 38 NHS tested, 19 demonstrated significant activity to MC dsDNA (defined as an OD380 > 1.0 at a 1:100 dilution of serum) without appreciable binding to dsDNA from Escherichia coli or calf thymus. In competition ELISA, antibodies in NHS to MC dsDNA showed equivalent inhibition by MC ssDNA or dsDNA. Antibody specificity was further evaluated by testing the effects of ionic strength on binding. By ELISA, antibodies in NHS had greater binding to MC dsDNA at high ionic strengths than those in systemic lupus erythematosus sera. These results indicate that NHS have antibodies which bind bacterial dsDNA and extend the range of DNA determinants that can be recognized as foreign by the normal immune system.
为评估人类对细菌双链（ds）DNA的抗体反应，通过酶联免疫吸附测定（ELISA）检测了正常人类受试者（NHS）血清与来自溶壁微球菌（MC）的高度纯化dsDNA的结合情况。在检测的38名NHS中，19名表现出对MC dsDNA的显著活性（定义为血清1:100稀释时OD380>1.0），而与大肠杆菌或小牛胸腺的dsDNA无明显结合。在竞争ELISA中，NHS中针对MC dsDNA的抗体对MC ssDNA或dsDNA表现出同等程度的抑制。通过检测离子强度对结合的影响进一步评估了抗体特异性。通过ELISA，NHS中的抗体在高离子强度下比系统性红斑狼疮血清中的抗体与MC dsDNA的结合更强。这些结果表明，NHS具有结合细菌dsDNA的抗体，并扩展了正常免疫系统可识别为外来的DNA决定簇范围。
