Yaspo M L, Crété N, Chettouh Z, Blouin J L, Rahmani Z, Stehelin D, Sinet P M, Créau-Goldberg N, Delabar J M
Laboratoire de Biochimie-Génétique URA 1335 CNRS, Hôpital Necker, Paris, France.
Hum Genet. 1992 Dec;90(4):427-34. doi: 10.1007/BF00220472.
To generate new chromosome 21 markers in a region that is critical for the pathogenesis of Down syndrome (D21S55-MX1), we used pulsed field gel electrophoresis (PFGE) to isolate a 600-kb NruI DNA fragment from the WA17 hybrid cell line, which has retained chromosome 21 as the only human material. This fragment, which contains the oncogene ETS2, was used to construct a partial genomic library. Among the 14 unique sequences that were isolated, 3 were polymorphic markers and contained sequences that are conserved in mammals. Five of these markers mapped on the ETS2-containing NruI fragment and allowed us to define an 800-kb high-resolution PFGE map.
为了在对唐氏综合征（D21S55-MX1）发病机制至关重要的区域生成新的21号染色体标记，我们使用脉冲场凝胶电泳（PFGE）从WA17杂交细胞系中分离出一个600 kb的NruI DNA片段，该细胞系仅保留了21号染色体作为唯一的人类物质。这个包含癌基因ETS2的片段被用于构建一个部分基因组文库。在分离出的14个独特序列中，有3个是多态性标记，并且包含在哺乳动物中保守的序列。其中5个标记定位在包含ETS2的NruI片段上，使我们能够定义一个800 kb的高分辨率PFGE图谱。
