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Improved control of partial DNA restriction enzyme digest in agarose using limiting concentrations of Mg++.

作者信息

Albertsen H M, Le Paslier D, Abderrahim H, Dausset J, Cann H, Cohen D

机构信息

Centre d'Etude du Polymorphisme Humain, Paris, France.

出版信息

Nucleic Acids Res. 1989 Jan 25;17(2):808. doi: 10.1093/nar/17.2.808.

DOI:10.1093/nar/17.2.808
PMID:2915934
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC331632/
Abstract
摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f53/331632/5076607e543d/nar00211-0336-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f53/331632/5076607e543d/nar00211-0336-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f53/331632/5076607e543d/nar00211-0336-a.jpg

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Improved control of partial DNA restriction enzyme digest in agarose using limiting concentrations of Mg++.使用极限浓度的Mg++改善琼脂糖中部分DNA限制性内切酶消化的控制。
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Rat hemoglobin interferes with DNA migration in agarose gels.大鼠血红蛋白会干扰DNA在琼脂糖凝胶中的迁移。
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Elution of DNA from agarose gels after electrophoresis.电泳后从琼脂糖凝胶中洗脱DNA。
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Scrambling of bands in gel electrophoresis of DNA.DNA凝胶电泳中条带的混乱。
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Efficient, small scale electroelution of high molecular weight DNA from agarose gels by a miniature vertical electrophoresis cell.使用微型垂直电泳槽从琼脂糖凝胶中高效小规模电洗脱高分子量DNA
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Pulsed-field gel electrophoresis.脉冲场凝胶电泳
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Construction of a large-insert yeast artificial chromosome library from sheep DNA.
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Human ureaplasmas show diverse genome sizes by pulsed-field electrophoresis.
Nucleic Acids Res. 1990 Mar 25;18(6):1451-5. doi: 10.1093/nar/18.6.1451.
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Streamlined approach to creating yeast artificial chromosome libraries from specialized cell sources.
Proc Natl Acad Sci U S A. 1990 Nov;87(21):8637-41. doi: 10.1073/pnas.87.21.8637.
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Methylase-limited partial NotI cleavage for physical mapping of genomic DNA.用于基因组DNA物理图谱绘制的甲基化酶限制的NotI部分酶切
Nucleic Acids Res. 1990 Jun 11;18(11):3287-91. doi: 10.1093/nar/18.11.3287.
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Construction and characterization of a yeast artificial chromosome library containing seven haploid human genome equivalents.包含七个单倍体人类基因组当量的酵母人工染色体文库的构建与表征。
Proc Natl Acad Sci U S A. 1990 Jun;87(11):4256-60. doi: 10.1073/pnas.87.11.4256.
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A test case for physical mapping of human genome by repetitive sequence fingerprints: construction of a physical map of a 420 kb YAC subcloned into cosmids.利用重复序列指纹图谱进行人类基因组物理图谱绘制的一个测试案例：构建亚克隆到黏粒中的420 kb酵母人工染色体的物理图谱。
Nucleic Acids Res. 1991 Feb 11;19(3):505-10. doi: 10.1093/nar/19.3.505.
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New chromosome 21 DNA markers isolated by pulsed field gel electrophoresis from an ETS2-containing Down syndrome chromosomal region.通过脉冲场凝胶电泳从含ETS2的唐氏综合征染色体区域分离出的新的21号染色体DNA标记。
Hum Genet. 1992 Dec;90(4):427-34. doi: 10.1007/BF00220472.