Michiels F, Burmeister M, Lehrach H
Science. 1987 Jun 5;236(4806):1305-8. doi: 10.1126/science.3035716.
The molecular analysis of genes identified by mutations is a major problem in mammalian genetics. As a step toward this goal, preparative field inversion gel electrophoresis (FIGE) was used to selectively isolate clones from the environment of genetically linked markers, and to select a subset of these clones containing sequences next to specific restriction sites rare in mammalian DNA. This approach has been used to generate a library highly enriched in sequences closely linked to the cystic fibrosis marker met. One clone derived from the end of a Not I restriction fragment containing the met sequence was analyzed in detail and localized within a long range map to a position 300 kilobase pairs 5' of the metD sequence.
对通过突变鉴定出的基因进行分子分析是哺乳动物遗传学中的一个主要问题。作为朝着这一目标迈出的一步，制备型场反转凝胶电泳（FIGE）被用于从遗传连锁标记的环境中选择性分离克隆，并从这些克隆中选择一部分包含在哺乳动物DNA中罕见的特定限制性位点附近序列的克隆。这种方法已被用于构建一个高度富集与囊性纤维化标记物met紧密连锁序列的文库。对一个源自包含met序列的Not I限制性片段末端的克隆进行了详细分析，并在一个长程图谱中定位到metD序列5'端300千碱基对的位置。
