Handa B K, Kay C
Department of Physical Chemistry, Roche Products Limited, Welwyn Garden City, Hertfordshire, England.
Int J Pept Protein Res. 1992 Nov;40(5):363-9. doi: 10.1111/j.1399-3011.1992.tb00312.x.
Two small peptide substrates for HIV-1 proteinase were synthesised. The sequences chosen were basically from that of the gag-pol protein, which is the natural substrate for the proteinase. To protect these peptides from the attack of exopeptidases, the N- and C-termini were suitably protected, which also makes these substrates specific to HIV-proteinase and eliminates the requirement for highly purified enzyme.
