Tadokoro K, Oki N, Fujii H, Ohshima A, Inoue T, Yamada M
National Children's Medical Research Center, Tokyo.
Jpn J Cancer Res. 1992 Nov;83(11):1198-203. doi: 10.1111/j.1349-7006.1992.tb02745.x.
We have analyzed the genomic structure of the human WT1 gene, one of the recessive oncogenes for Wilms' tumor at chromosome 11p13. By analyses of three cosmids covering the WT1 gene as well as products generated by polymerase chain reaction, cleavage sites for 10 restriction enzymes were mapped in a region of about 80 kb, and the positions of 10 exons were defined. We also mapped two polymorphic sites for TaqI. Our genomic map will be useful to analyze DNA abnormalities sometimes found in the tumors, as well as loss of heterozygosity.
我们分析了人类WT1基因的基因组结构，该基因是位于11号染色体p13区的肾母细胞瘤隐性致癌基因之一。通过对覆盖WT1基因的三个黏粒以及聚合酶链反应产生的产物进行分析，在约80 kb的区域内绘制了10种限制性内切酶的切割位点，并确定了10个外显子的位置。我们还绘制了TaqI的两个多态性位点。我们的基因组图谱将有助于分析肿瘤中有时发现的DNA异常以及杂合性缺失。
