大肠杆菌染色体DNA的nrdA - nrdB - ftsB - glpT区域的物理图谱。

Physical map of the nrdA-nrdB-ftsB-glpT region of the chromosomal DNA of Escherichia coli.

作者信息

Yamada M, Takeda Y, Okamoto K, Hirota Y

出版信息

Gene. 1982 Jun;18(3):309-18. doi: 10.1016/0378-1119(82)90169-x.

Abstract

Seven pLC plasmids (pLC 3-46, 8-12, 8-24, 8-29, 14-12, 19-24 and 42-17) which complemented nrdA, nrdB, ftsB and/or glpT mutations of Escherichia coli were analyzed. A restriction map of each plasmid was constructed and restriction fragments were subcloned into pBR322. A physical map of approx. a 15 X 10(6) Mr segment of the chromosomal DNA was deduced from the overlapping region of the pLC plasmids. The pLC plasmids and newly constructed plasmids were examined for the ability to rescue the mutations. The complementation tests defined the location of the genes in the 15 X 10(6) Mr segment in the following order: nrdA-nrdB-ftsB-glpT. Functional nrdAB and ftsB genes were located in the 3.1 X 10(6) Mr EcoRI-PstI fragment.

摘要

对7种可互补大肠杆菌nrdA、nrdB、ftsB和/或glpT突变的pLC质粒（pLC 3 - 46、8 - 12、8 - 24、8 - 29、14 - 12、19 - 24和42 - 17）进行了分析。构建了每个质粒的限制性图谱，并将限制性片段亚克隆到pBR322中。从pLC质粒的重叠区域推导出了约15×10⁶ Mr的染色体DNA片段的物理图谱。检测了pLC质粒和新构建的质粒拯救突变的能力。互补试验按以下顺序确定了基因在15×10⁶ Mr片段中的位置：nrdA - nrdB - ftsB - glpT。功能性nrdAB和ftsB基因位于3.1×10⁶ Mr的EcoRI - PstI片段中。