通过三色荧光原位杂交对跨越包含肾细胞癌肿瘤抑制基因区域的三个多态性DNA标记进行物理排序。
Physical ordering of three polymorphic DNA markers spanning the regions containing a tumor suppressor gene of renal cell carcinoma by three-color fluorescent in situ hybridization.
作者信息
Inazawa J, Ariyama T, Abe T
机构信息
Department of Hygiene, Kyoto Prefectural University of Medicine.
出版信息
Jpn J Cancer Res. 1992 Dec;83(12):1248-52. doi: 10.1111/j.1349-7006.1992.tb02753.x.
Abstract
Fluorescent in situ hybridization (FISH) is a powerful technique for gene mapping and multi-color FISH allows us to determine directly the order of two or more probes on both metaphase and interphase chromosomes. We report the physical ordering of three DNA markers by three-color FISH using two fluorochrome dyes, fluorescein isothiocyanate (FITC; green) and rhodamine (red). The third color was visualized as a pseudo-color (yellow) generated by optical interference with FITC and rhodamine. Using this system we could rapidly determine the order of three polymorphic DNA markers located on the 3p23-p21.2 bands which span a tumor suppressor gene for renal cell carcinoma, lung carcinoma, and several other types of tumors.
摘要
荧光原位杂交（FISH）是一种用于基因定位的强大技术，多色FISH使我们能够直接确定中期和间期染色体上两个或更多探针的顺序。我们报告了使用两种荧光染料，即异硫氰酸荧光素（FITC；绿色）和罗丹明（红色），通过三色FISH对三个DNA标记进行物理排序。第三种颜色通过与FITC和罗丹明的光学干涉产生的伪彩色（黄色）显现出来。利用该系统，我们能够快速确定位于3p23-p21.2带的三个多态性DNA标记的顺序，该区域跨越了肾细胞癌、肺癌和其他几种肿瘤类型的一个肿瘤抑制基因。
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