Okano K, Miyajima N, Takada N, Kobayashi M, Maekawa H
Department of Agrobiology, Faculty of Agriculture, University of Tokyo, Japan.
In Vitro Cell Dev Biol. 1992 Nov-Dec;28A(11-12):779-81. doi: 10.1007/BF02631067.
We established basic conditions for transient gene expression and selection of antibiotics in the cultured cell line of silkworm, Bombyx mori, by use of the promoter of the heat shock protein (hsp70) gene of Drosophila melanogaster. The control promoter (hsp70) promoted the expression of chloramphenicol acetyltransferase (CAT) gene ligated at the downstream, dependent on the orientation of the promoter in the silkworm cell. The cell line is able to be supplied for the promoter assay of the silkworm genes. The concentration for the drug selection was determined as 0.75 mg/ml on neomycin analog, G418 (geneticin).
我们利用黑腹果蝇热休克蛋白（hsp70）基因的启动子，在家蚕培养细胞系中建立了瞬时基因表达和抗生素选择的基本条件。对照启动子（hsp70）促进了连接在下游的氯霉素乙酰转移酶（CAT）基因的表达，这取决于启动子在家蚕细胞中的方向。该细胞系可用于家蚕基因的启动子分析。新霉素类似物G418（遗传霉素）的药物选择浓度确定为0.75 mg/ml。
