Mascher H, Kikuta C, Metz R, Vergin H
Pharm-Analyt Labor GmbH, Traiskirchen, Austria.
J Chromatogr. 1992 Nov 27;583(1):122-7. doi: 10.1016/0378-4347(92)80353-r.
A high-performance liquid chromatographic method for the determination of acyclovir in human plasma has been developed. It is the first published chromatographic method capable of determining acyclovir in plasma with sufficient sensitivity and for sufficiently long periods of time following oral administration of a standard dose of acyclovir during pharmacokinetic investigations. Following precipitations of the proteins with perchloric acid, the sample is chromatographed with a strongly acidic mobile phase on a reversed-phase column, and is then subjected to fluorometric detection (excitation 260 nm, emission 375 nm). The determination limit is 6-10 ng/ml human plasma. The calibration is linear in the range 10-12,400 ng/ml plasma, with the coefficients of variation less than 8%. The absolute recovery rate is between 102 and 113%. This method has already been used to analyse several thousand plasma samples.
已开发出一种用于测定人血浆中阿昔洛韦的高效液相色谱法。这是首次发表的色谱方法，能够在药代动力学研究中口服标准剂量阿昔洛韦后，以足够的灵敏度和足够长的时间测定血浆中的阿昔洛韦。用高氯酸沉淀蛋白质后，样品在反相柱上用强酸性流动相进行色谱分析，然后进行荧光检测（激发波长260nm，发射波长375nm）。测定限为人血浆6 - 10ng/ml。校准曲线在血浆浓度10 - 12400ng/ml范围内呈线性，变异系数小于8%。绝对回收率在102%至113%之间。该方法已用于分析数千份血浆样本。
