Swart K J, Hundt H K, Groenewald A M
Department of Pharmacology (G6), UOFS, Bloemfontein, South Africa.
J Chromatogr A. 1994 Mar 4;663(1):65-9. doi: 10.1016/0021-9673(94)80496-6.
Owing to the low plasma concentrations of acyclovir obtained during pharmacokinetic studies after low-dosage oral administration, a sensitive, automated HPLC method had to be developed for determining acyclovir in a large number of plasma samples. Extraction and injection of the samples were done automatically by a Gilson ASPEC system using tC18, 100-mg Sep-Pak Vac extraction columns. The extracts were chromatographed on a Nova-Pak C18 column with sodium octanesulphonate and methanol in the mobile phase. The analyte was detected at 250 nm. The calibration graphs were linear up to at least 1200 ng/ml and the limit of quantification was 10 ng/ml.
由于在低剂量口服给药后的药代动力学研究中获得的阿昔洛韦血浆浓度较低，因此必须开发一种灵敏的自动化高效液相色谱法来测定大量血浆样本中的阿昔洛韦。使用100毫克的tC18 Sep-Pak Vac萃取柱，通过吉尔森ASPEC系统自动完成样品的萃取和进样。萃取物在Nova-Pak C18柱上进行色谱分析，流动相为辛烷磺酸钠和甲醇。在250纳米处检测分析物。校准曲线在至少1200纳克/毫升范围内呈线性，定量限为10纳克/毫升。
