气相色谱法测定人血清中三甲双酮及其去甲基代谢物二甲双酮的改进方法

Improved method for the determination of trimethadione and its demethylated metabolite, dimethadione, in human serum by gas chromatography.

作者信息

Tanaka E, Misawa S

机构信息

Institute of Community Medicine, University of Tsukuba, Ibaraki-ken, Japan.

出版信息

J Chromatogr. 1992 Dec 23;584(2):267-9. doi: 10.1016/0378-4347(92)80585-e.

DOI:10.1016/0378-4347(92)80585-e

PMID:1484112

Abstract

An improved gas chromatographic method, involving the use of a wide-bore capillary column, for the determination of trimethadione and its only demethylated metabolite, dimethadione, in human serum is described. The results indicate that both substances and the internal standard (maleinimide) were well separated with no tailing peak. The detection limit was 10 ng/ml for trimethadione and 50 ng/ml for dimethadione. This improved method is reliable in terms of sensitivity, selectivity and reproducibility for the simultaneous determination of both compounds in human serum.

摘要

描述了一种改进的气相色谱法，该方法使用宽口径毛细管柱测定人血清中的三甲双酮及其唯一的去甲基代谢物二甲双酮。结果表明，两种物质与内标（马来酰亚胺）分离良好，无拖尾峰。三甲双酮的检测限为10 ng/ml，二甲双酮的检测限为50 ng/ml。这种改进的方法在同时测定人血清中两种化合物的灵敏度、选择性和重现性方面是可靠的。