Kurohmaru M, Kanai Y, Hayashi Y
Department of Veterinary Anatomy, Faculty of Agriculture, University of Tokyo, Japan.
Tissue Cell. 1992;24(6):895-903. doi: 10.1016/0040-8166(92)90024-2.
The distribution of F-actin and intermediate filaments in the W/WV mouse was investigated by light and transmission electron microscopy, and fluorescence methods. No spermatogenic cells were detected in the seminiferous epithelium of the W/WV mouse. Its seminiferous tubule was one-half the diameter of that in the normal (+/+) mouse. The Sertoli cell which was an only component of the W/WV mouse seminiferous epithelium was decreased in height, but still retained the polarity as evidenced by light microscopy. The Sertoli cell organelles were similar in appearance when normal and mutant mice were compared. F-actin was recognized at ectoplasmic specialization (ES) of the W/WV mouse Sertoli cell and appeared similar to the normal mouse. However, the junction with ES was more extensive compared with that of the normal mouse Vimentin in the W/WV mouse Sertoli cell was distributed around the nucleus and extended towards the tubular lumen similar to the normal mouse. Its extension within the Sertoli cell trunk, however, was restricted to a lesser degree as compared with that in the normal. Thus, the subcellular Sertoli cell and the distribution of F-actin and intermediate filaments (vimentin) in the W/WV mouse Sertoli cell seemed not to be strikingly affected by lack of spermatogenic cells, suggesting minimal influence of germ cells on Sertoli cell cytology and cytoskeleton.
通过光学显微镜、透射电子显微镜和荧光方法研究了W/WV小鼠中F-肌动蛋白和中间丝的分布。在W/WV小鼠的生精上皮中未检测到生精细胞。其生精小管直径是正常（+/+）小鼠的一半。作为W/WV小鼠生精上皮唯一成分的支持细胞高度降低，但通过光学显微镜观察仍保留极性。比较正常小鼠和突变小鼠时，支持细胞的细胞器外观相似。在W/WV小鼠支持细胞的外质特化（ES）处可识别出F-肌动蛋白，其与正常小鼠相似。然而，与正常小鼠相比，W/WV小鼠支持细胞中与ES的连接更为广泛。W/WV小鼠支持细胞中的波形蛋白分布在细胞核周围并向管腔延伸，与正常小鼠相似。然而，与正常情况相比，其在支持细胞主干内的延伸受到的限制较小。因此，W/WV小鼠支持细胞的亚细胞结构以及支持细胞中F-肌动蛋白和中间丝（波形蛋白）的分布似乎并未受到生精细胞缺乏的显著影响，这表明生殖细胞对支持细胞细胞学和细胞骨架的影响最小。
