Steger K, Wrobel K H
Institute of Anatomy, University of Regensburg, Germany.
Anat Embryol (Berl). 1994 Jun;189(6):521-30. doi: 10.1007/BF00186825.
The distribution pattern of actin, desmin, vimentin and tubulin in the ovine testis during postnatal development was investigated by means of immunohistochemical methods. The postnatal development of the ovine testis can be divided into five phases. Phases I through III represent the prepubertal period, phase IV puberty and phase V the postpubertal adult stage. In peritubular cells alpha-smooth muscle actin is present, its amount increasing with advancing age of the animals. Structural F-actin is localized in peritubular myoid cells and Sertoli cells, of the adult testis. In Sertoli cells structural F-actin-positive material is observed at the level of the Sertoli-Sertoli junctions, at contact sites of Sertoli cells with primary spermatocytes and in the immediate vicinity of elongating spermatid heads during the acrosome phase of spermiogenesis. Desmin is present in intertubular and peritubular cells during the early prepubertal period, but vanishes completely as soon as the animals reach puberty. Vimentin is present in the cytoplasm of prespermatogonia I, but disappears when these change into prespermatogonia II. In prepubertal supporting cells the vimentin content increases, and in the adult the positive filament bundles create a flame-like pattern around the unstained nucleus. Cyclical variations during the seminiferous epithelial cycle are not observed. Expression of alpha-tubulin is found in the cytoplasm of prespermatogonia I and to a lesser extent in prespermatogonia II and spermatogonia. The immunoreaction is also seen in the microtubules of the axonema and manchette of elongating spermatids. The histochemical demonstration of the high alpha-tubulin concentration in supporting and Sertoli cells is an excellent method for studying changes of cellular shape and size during ontogenesis as well as during the seminiferous epithelial cycle.
采用免疫组织化学方法研究了绵羊睾丸生后发育过程中肌动蛋白、结蛋白、波形蛋白和微管蛋白的分布模式。绵羊睾丸的生后发育可分为五个阶段。第一阶段至第三阶段代表青春期前时期，第四阶段为青春期，第五阶段为青春期后的成年期。在睾丸外周细胞中存在α-平滑肌肌动蛋白，其含量随动物年龄增长而增加。成年睾丸的睾丸外周肌样细胞和支持细胞中存在结构型F-肌动蛋白。在支持细胞中，在支持细胞-支持细胞连接水平、支持细胞与初级精母细胞的接触部位以及精子发生顶体期伸长的精子头部紧邻区域观察到结构型F-肌动蛋白阳性物质。在青春期前早期，结蛋白存在于管间细胞和睾丸外周细胞中，但动物一进入青春期就完全消失。波形蛋白存在于I型精原细胞前体细胞的细胞质中，但当这些细胞转变为II型精原细胞前体细胞时消失。在青春期前的支持细胞中波形蛋白含量增加，在成年期，阳性细丝束在未染色的细胞核周围形成火焰状图案。未观察到生精上皮周期中的周期性变化。α-微管蛋白在I型精原细胞前体细胞的细胞质中表达，在II型精原细胞前体细胞和精原细胞中表达较少。在伸长精子的轴丝和袖套微管中也可见免疫反应。支持细胞和支持细胞中高浓度α-微管蛋白的组织化学显示是研究个体发育过程中以及生精上皮周期中细胞形状和大小变化的一种极好方法。
