Aumüller G, Steinbrück M, Krause W, Wagner H J
Department of Anatomy and Cell Biology, Philipps-Universität Marburg, Federal Republic of Germany.
Anat Embryol (Berl). 1988;178(2):129-36. doi: 10.1007/BF02463646.
The presence, distribution and spatial arrangement of vimentin-type intermediate filaments in Sertoli cells from human testis biopsies, were studied in semithin and ultrathin sections using a polyclonal rabbit antiserum. At the ultrastructural level, vimentin immunoreactivity was seen concentrated around the nuclei, along fibrillary material within the cytoplasm and at the ectoplasmic specializations of the Sertoli cell junctions, as well as throughout the periphery of the Sertoli cell processes. It is therefore well suited as a marker for Sertoli cell configuration. In computer-aided 3D reconstructions of 20 serial sections, Sertoli cells displayed particular configurations of intermediate filaments in the different stages of spermatogenesis. Two basic configurations, named AS (before spermiation, stages V, VI, I and II), and PS (after spermiation, stages III and IV) respectively, could be differentiated. In addition to the reconstruction and morphological analysis of vimentin filaments in Sertoli cells from patients with unaltered spermatogenesis (obstructive azoospermia), pathological specimens (spermatogenetic arrest, Sertoli cells only-syndrome) were studied with respect to vimentin immunohistochemistry. The results indicate that vimentin filaments play an important role in the adaptation of Sertoli cells to the varying configurations of neighbouring cells during spermatogenesis as well as under pathological conditions.
利用兔多克隆抗血清，在半薄切片和超薄切片中研究了人睾丸活检组织中支持细胞波形蛋白型中间丝的存在、分布及空间排列。在超微结构水平上，波形蛋白免疫反应性可见于细胞核周围、细胞质内的纤维状物质处、支持细胞连接的外质特化部位以及支持细胞突起的整个周边。因此，它非常适合作为支持细胞形态的标志物。在对20个连续切片进行的计算机辅助三维重建中，支持细胞在精子发生的不同阶段呈现出中间丝的特定形态。可以区分出两种基本形态，分别命名为AS（精子释放前，V、VI、I和II期）和PS（精子释放后，III和IV期）。除了对精子发生正常（梗阻性无精子症）患者的支持细胞中波形蛋白丝进行重建和形态分析外，还对病理标本（精子发生停滞、唯支持细胞综合征）进行了波形蛋白免疫组化研究。结果表明，波形蛋白丝在精子发生过程中以及病理条件下，在支持细胞适应相邻细胞的不同形态方面发挥着重要作用。
