Immunoaffinity-chromatography purification of salbutamol in liver and HPLC-fluorometric detection at trace residue level.
作者信息
Degroodt J M, Wyhowski de Bukanski B, Srebrnik S
机构信息
Ministère de la Santé Publique et de l'Environnement, Bruxelles, Belgium.
出版信息
Z Lebensm Unters Forsch. 1992 Dec;195(6):566-8. doi: 10.1007/BF01204565.
PMID:1485484
Abstract
A method combining immunoaffinity-chromatography (IAC) and high-performance liquid chromatography (HPLC) for the analysis of Salbutamol in liver with a low quantification limit of 1 micrograms/kg has been developed. Salbutamol was extracted with 0.01 mol/L HCl and purified by IAC. The samples were analysed on a liquid chromatograph fitted with a C18 mu-Bondapak column. A fluorometer was used for the detection of salbutamol. Recoveries of 67-80% could be obtained.
摘要
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