Boyd D, O'Keeffe M, Smyth M R
National Food Centre, Teagasc, Dunsinea, Dublin, Ireland.
Analyst. 1994 Jul;119(7):1467-70. doi: 10.1039/an9941901467.
A procedure for the extraction of salbutamol including conjugated forms of the drug, from liver samples, is described. It combines matrix solid-phase dispersion with radioimmunoassay for the measurement of salbutamol residues at the sub-ppb level. Inter- and intra-assay validation, carried out on fortified liver samples, show good recoveries over the range 1-5 ppb of salbutamol. An enzyme hydrolysis procedure was optimized for the deconjugation of incurred residue. The developed procedure is shown to be suitable for the extraction and determination of other beta-agonists such as clenbuterol, mabuterol, terbutaline, and cimaterol at residue levels of less than 1 ng g-1.
本文描述了一种从肝脏样本中提取沙丁胺醇（包括药物结合形式）的方法。该方法将基质固相分散与放射免疫分析相结合，用于测定亚纳克/克水平的沙丁胺醇残留。在加标肝脏样本上进行的批间和批内验证表明，在1-5 ppb的沙丁胺醇范围内具有良好的回收率。对酶水解程序进行了优化，以实现对残留结合物的解结合。所开发的方法被证明适用于提取和测定其他β-激动剂，如克伦特罗、马布特罗、特布他林和西马特罗，残留水平低于1 ng g-1。
