Slemons R D, Swayne D E
Department of Veterinary Preventive Medicine, College of Veterinary Medicine, Ohio State University, Columbus 43210.
Avian Dis. 1992 Oct-Dec;36(4):926-31.
Tissue tropism properties of A/chicken/Alabama/75 (H4N8) were examined after intravenous inoculation of 5-week-old specific-pathogen-free chickens. From 14 clinically normal chickens euthanatized on days 1-20 postinoculation, the frequencies of virus recovery were highest for cloacal swabs (86%), bursal swabs (64%), and kidney tissues (64%) and lowest for tracheal swabs (14%), thymus tissues (14%), bone-marrow swabs (7%), and brain tissues (0%). Evidence that the high frequency of virus recovery from kidney tissues was associated with virus replication in the kidney tissues was provided by high virus titers, ranging up to 10(9.5) mean embryo infectious dose per gram of kidney tissue, and by identification of intranuclear and intracytoplasmic type A influenza nucleoprotein in kidney cells using immunohistochemistry. Virus-recovery and virus titer results from three chickens that died on days 4 and 5 postinoculation paralleled the results from the clinically normal chickens. These findings indicate that A/chicken/Alabama/75 has nephrotropic properties similar to nephrotropic properties previously reported for waterfowl-origin type A influenza viruses and provide evidence that kidney lesions could be manifestations of systemic influenza infections in commercial laying chickens.
在对5周龄无特定病原体的鸡进行静脉接种后，检测了A/鸡/阿拉巴马/75（H4N8）的组织嗜性特性。在接种后第1至20天对14只临床正常的鸡实施安乐死，泄殖腔拭子（86%）、法氏囊拭子（64%）和肾脏组织（64%）的病毒检出频率最高，气管拭子（14%）、胸腺组织（14%）、骨髓拭子（7%）和脑组织（0%）的病毒检出频率最低。高病毒滴度（每克肾脏组织高达10(9.5)平均胚胎感染剂量）以及使用免疫组织化学在肾细胞中鉴定出核内和胞质内甲型流感核蛋白，为从肾脏组织中高频率检出病毒与病毒在肾脏组织中的复制相关提供了证据。接种后第4天和第5天死亡的3只鸡的病毒检出和病毒滴度结果与临床正常鸡的结果相似。这些发现表明，A/鸡/阿拉巴马/75具有与先前报道的水禽源甲型流感病毒相似肾嗜性特性，并提供了证据表明肾脏病变可能是商品蛋鸡全身性流感感染的表现。
