Ebbecke M, Unterberg C, Buchwald A, Stöhr S, Wiegand V
Department of Internal Medicine, University of Göttingen, FRG.
Basic Res Cardiol. 1992 Nov-Dec;87(6):585-91. doi: 10.1007/BF00788668.
The effects of a c-myc antisense phosphorothioate DNA oligonucleotide were assessed on the proliferation rate of human arterial smooth muscle cells (HSMCs). Compared to a control oligonucleotide the antisense oligonucleotide suppressed the proliferation of HSMCs in a concentration-dependent manner without a major cytotoxic effect. Outgrowth of HSMCs from media explants was significantly inhibited as well. Induction of c-myc expression by serum stimulation of cells was blunted by the antisense oligonucleotide, as shown by immunoblotting. These results demonstrate that c-myc expression is an essential factor for proliferation of HSMCs after growth stimulation, and they show the potential of antisense technology for modulating gene expression of HSMCs in vitro.
评估了一种c-myc反义硫代磷酸酯DNA寡核苷酸对人动脉平滑肌细胞（HSMCs）增殖速率的影响。与对照寡核苷酸相比，反义寡核苷酸以浓度依赖性方式抑制HSMCs的增殖，且无明显细胞毒性作用。培养基外植体中HSMCs的生长也受到显著抑制。免疫印迹显示，反义寡核苷酸可减弱血清刺激细胞诱导的c-myc表达。这些结果表明，c-myc表达是生长刺激后HSMCs增殖的一个重要因素，并且显示了反义技术在体外调节HSMCs基因表达的潜力。
