Ross R
J Cell Biol. 1971 Jul;50(1):172-86. doi: 10.1083/jcb.50.1.172.
Smooth muscle derived from the inner media and intima of immature guinea pig aorta were grown for up to 8 wk in cell culture. The cells maintained the morphology of smooth muscle at all phases of their growth in culture. After growing to confluency, they grew in multiple overlapping layers. By 4 wk in culture, microfibrils (110 A) appeared within the spaces between the layers of cells. Basement membrane-like material also appeared adjacent to the cells. Analysis of the microfibrils showed that they have an amino acid composition similar to that of the microfibrillar protein of the intact elastic fiber. These investigations coupled with the radioautographic observations of the ability of aortic smooth muscle to synthesize and secrete extracellular proteins demonstrate that this cell is a connective tissue synthetic cell.
从幼年豚鼠主动脉中膜和内膜分离出的平滑肌细胞在细胞培养中生长长达8周。这些细胞在培养的各个生长阶段都保持着平滑肌的形态。细胞长满后，会形成多层重叠生长。培养4周后，在细胞层之间的间隙中出现了微原纤维（110埃）。细胞附近还出现了类似基底膜的物质。对微原纤维的分析表明，它们的氨基酸组成与完整弹性纤维的微原纤维蛋白相似。这些研究以及对主动脉平滑肌合成和分泌细胞外蛋白能力的放射自显影观察表明，这种细胞是一种结缔组织合成细胞。
