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通过脱氧核苷H-膦酸酯中间体合成DNA。

Synthesis of DNA via deoxynucleoside H-phosphonate intermediates.

作者信息

Froehler B C, Ng P G, Matteucci M D

出版信息

Nucleic Acids Res. 1986 Jul 11;14(13):5399-407. doi: 10.1093/nar/14.13.5399.

DOI:10.1093/nar/14.13.5399
PMID:3737406
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC311548/
Abstract

Deoxynucleoside H-phosphonates are used in the chemical synthesis of deoxyoligonucleotides up to 107 bases in length. The biological activity of the synthetic DNA is assessed by cloning into M13 and sequencing. An improved synthesis of protected deoxynucleoside H-phosphonates is also described.

摘要

脱氧核苷H-膦酸酯用于化学合成长度达107个碱基的脱氧寡核苷酸。通过克隆到M13中并进行测序来评估合成DNA的生物活性。本文还描述了一种改进的受保护脱氧核苷H-膦酸酯的合成方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab54/311548/dbd63c9c505d/nar00282-0285-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab54/311548/2ddbcb86f069/nar00282-0283-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab54/311548/dbd63c9c505d/nar00282-0285-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab54/311548/2ddbcb86f069/nar00282-0283-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab54/311548/dbd63c9c505d/nar00282-0285-a.jpg

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本文引用的文献

1
Syringe method for stepwise chemical synthesis of oligonucleotides.用于寡核苷酸逐步化学合成的注射器法
Nucleic Acids Res. 1982 May 25;10(10):3249-60. doi: 10.1093/nar/10.10.3249.
2
Dialkylformamidines: depurination resistant N6-protecting group for deoxyadenosine.二烷基甲脒：用于脱氧腺苷的抗脱嘌呤N6保护基团。
Nucleic Acids Res. 1983 Nov 25;11(22):8031-6. doi: 10.1093/nar/11.22.8031.
3
Modification of guanine bases by nucleoside phosphoramidite reagents during the solid phase synthesis of oligonucleotides.在寡核苷酸固相合成过程中，鸟嘌呤碱基被核苷亚磷酰胺试剂修饰。
硫代膦酸酯/硫代磷酸酯和磷酰胺/磷酰胺寡核苷酸的固相合成。
Molecules. 2019 May 15;24(10):1872. doi: 10.3390/molecules24101872.
4
Improved strategy for large scale isolation of sialylglycopeptide (SGP) from egg yolk powder.从蛋黄粉中大规模分离唾液酸糖肽（SGP）的改进策略。
MethodsX. 2019 Apr 9;6:773-778. doi: 10.1016/j.mex.2019.04.007. eCollection 2019.
5
Synthesis of oligonucleotides on a soluble support.在可溶性载体上合成寡核苷酸。
Beilstein J Org Chem. 2017 Jul 12;13:1368-1387. doi: 10.3762/bjoc.13.134. eCollection 2017.
6
Synthesis of DNA/RNA and their analogs via phosphoramidite and H-phosphonate chemistries.通过亚磷酰胺和 H-膦酸酯化学合成 DNA/RNA 及其类似物。
Molecules. 2013 Nov 18;18(11):14268-84. doi: 10.3390/molecules181114268.
7
New access to H-phosphonates via metal-catalyzed phosphorus-oxygen bond formation.通过金属催化形成磷-氧键实现对H-膦酸酯的新合成方法。
Tetrahedron Lett. 2007 Sep 10;48(37):6505-6508. doi: 10.1016/j.tetlet.2007.07.057.
8
Recombinant decorsin: dynamics of the RGD recognition site.重组去整合素：RGD识别位点的动力学
Protein Sci. 2000 Aug;9(8):1428-38. doi: 10.1110/ps.9.8.1428.
9
Targeted elimination of zygotic messages in Xenopus laevis embryos by modified oligonucleotides possessing terminal cationic linkages.通过具有末端阳离子连接的修饰寡核苷酸在非洲爪蟾胚胎中靶向消除合子信息。
Nucleic Acids Res. 2000 May 15;28(10):2153-7. doi: 10.1093/nar/28.10.2153.
10
Understanding oligonucleotide-mediated inhibition of gene expression in Xenopus laevis oocytes.了解寡核苷酸介导的非洲爪蟾卵母细胞基因表达抑制作用。
Nucleic Acids Res. 2000 Mar 1;28(5):1154-61. doi: 10.1093/nar/28.5.1154.
Nucleic Acids Res. 1985 Sep 25;13(18):6447-65. doi: 10.1093/nar/13.18.6447.
4
DNA sequencing with chain-terminating inhibitors.使用链终止抑制剂的DNA测序。
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