Kaufhold A, Livdahl C, Ferrieri P
University of Minnesota Medical School, Department of Laboratory Medicine and Pathology, Minneapolis 55455.
Zentralbl Bakteriol. 1992 Oct;277(3):309-19. doi: 10.1016/s0934-8840(11)80908-4.
Two groups of clinical Staphylococcus aureus isolates were examined by molecular typing methods. Detailed protocols for the technical procedures are described. By means of plasmid DNA profiles, plasmid DNA restriction enzyme digestion, as well as whole-cell DNA endonuclease digestions subjected to conventional agarose gel electrophoresis and pulsed-field gel electrophoresis, eight methicillin-susceptible S. aureus strains from three patients segregated into three different groups. Consecutive isolates from the same patient revealed identical fingerprints. Generally, good agreement between different molecular typing methods also was achieved in the investigation of 28 methicillin-resistant isolates. However, compared to whole-cell DNA restriction endonuclease analysis, plasmid DNA profiling (all but one strain had detectable plasmid DNA) showed a somewhat greater discriminatory ability. The techniques evaluated were reproducible and relatively easy to perform and provided valuable tools for studying the epidemiology of S. aureus in hospitals.
通过分子分型方法对两组临床金黄色葡萄球菌分离株进行了检测。文中描述了技术操作的详细方案。借助质粒DNA图谱、质粒DNA限制性内切酶消化，以及全细胞DNA内切酶消化后进行常规琼脂糖凝胶电泳和脉冲场凝胶电泳，来自三名患者的八株甲氧西林敏感金黄色葡萄球菌菌株被分为三个不同的组。同一患者的连续分离株显示出相同的指纹图谱。总体而言，在对28株耐甲氧西林分离株的研究中，不同分子分型方法之间也取得了较好的一致性。然而，与全细胞DNA限制性内切酶分析相比，质粒DNA图谱分析（除一株菌株外所有菌株均可检测到质粒DNA）显示出更强的鉴别能力。所评估的技术具有可重复性且相对易于操作，为研究医院内金黄色葡萄球菌的流行病学提供了有价值的工具。
