Liu P Y, Shi Z Y, Lau Y J, Hu B S, Shyr J M, Tsai W S, Lin Y H, Tseng C Y
Section of Infectious Diseases, Taichung Veterans General Hospital, Taiwan, Republic of China.
Clin Infect Dis. 1996 Jan;22(1):86-90. doi: 10.1093/clinids/22.1.86.
We used restriction endonuclease analysis of plasmids (REAP) and pulsed-field gel electrophoresis (PFGE) to investigate clusterings of methicillin-resistant Staphylococcus aureus (MRSA) infections in our orthopedic unit, neurosurgery unit, intensive care unit, and burn unit. Fourteen different strain types were identified by REAP and 10 different strain types were identified by PFGE among 25 MRSA isolates collected during these incidents of infection. Though neither technique was clearly superior to the other for typing MRSA isolates, REAP is recommended as a relatively simple and reproducible technique for the preliminary investigation of MRSA infection outbreaks in clinical settings.
我们采用质粒限制性内切酶分析（REAP）和脉冲场凝胶电泳（PFGE）来研究我院骨科、神经外科、重症监护病房和烧伤病房耐甲氧西林金黄色葡萄球菌（MRSA）感染的聚集情况。在这些感染事件期间收集的25株MRSA分离株中，通过REAP鉴定出14种不同的菌株类型，通过PFGE鉴定出10种不同的菌株类型。虽然两种技术在对MRSA分离株进行分型时都没有明显优于对方，但REAP作为一种相对简单且可重复的技术，推荐用于临床环境中MRSA感染暴发的初步调查。
