Springer W D, Srb A M
Proc Natl Acad Sci U S A. 1978 Mar;75(3):1461-5. doi: 10.1073/pnas.75.3.1461.
A procedure using ion exchange chromatography has been developed to detect alterations in a polysaccharide produced by Neurospora crassa. The polysaccharide, isolated from medium that has supported the growth of a culture, is highly responsive to the 3-methyl-2-benzothiazolinone hydrazone assay, indicating a high hexosamine content. The substance elaborated by wild-type N. crassa can be fractionated into two components that appear by rechromatography to be closely related. When isolated from mutants of the peak (pk) locus, the corresponding polysaccharide cannot be resolved into two components. Instead, a single component is consistently found. This variant chromatographic pattern cosegregates with morphological effects of the pk allele after crosses with the wild type. The polysaccharide isolated from a wild-type culture that has been induced by sorbose to phenocopy the hyphal characteristics of pk mutants elutes from the ion exchange column in a manner similar to the corresponding polysaccharide from the pk mutants.
已开发出一种使用离子交换色谱法的程序来检测粗糙脉孢菌产生的多糖的变化。从支持培养物生长的培养基中分离出的多糖对3-甲基-2-苯并噻唑啉酮腙测定法高度敏感，表明其己糖胺含量很高。野生型粗糙脉孢菌产生的物质可分为两个组分，经再色谱分析后显示它们密切相关。当从峰（pk）位点的突变体中分离时，相应的多糖无法分解为两个组分。相反，始终只发现一个组分。这种变异的色谱模式在与野生型杂交后与pk等位基因的形态学效应共分离。从已被山梨糖诱导以模拟pk突变体菌丝特征的野生型培养物中分离出的多糖，从离子交换柱上洗脱的方式与来自pk突变体的相应多糖相似。
