Herrero-Herrero J I, Walker D H, Ruiz-Beltrán R
Department of Medicine, University Hospital, Paseo de San Vicente, Salmanca, Spain.
Eur J Clin Microbiol Infect Dis. 1992 Oct;11(10):939-42. doi: 10.1007/BF01962380.
The reactivity of antigenic components of Rickettsia conorii with sequentially obtained sera from 20 adult Spanish patients with Mediterranean spotted fever was analyzed by Western blot. The major rickettsial antigens reacting with the serum samples corresponded to molecular weights of 135 and 115 kDa. These antigens constantly exhibited higher staining intensity than the other antigens, and reacted with 100% and 86.7%, respectively, of acute sera and with 100% of convalescent phase samples. Rickettsial lipopolysaccharide antigens reacted with 94.7% of sera collected in the fourth and fifth week after onset of symptoms. Other major antigens reactive in the blots had molecular sizes of 160, 100, 90 and 60 kDa, and a relatively frequent humoral immune response was also seen to antigens of 80, 73 and 55 kDa.
通过蛋白质免疫印迹法分析了康氏立克次体抗原成分与20例患地中海斑疹热的成年西班牙患者序贯采集的血清之间的反应性。与血清样本发生反应的主要立克次体抗原的分子量分别为135 kDa和115 kDa。这些抗原的染色强度始终高于其他抗原，分别与100%的急性期血清和86.7%的急性期血清以及100%的恢复期样本发生反应。立克次体脂多糖抗原与症状出现后第四周和第五周采集的94.7%的血清发生反应。免疫印迹中其他具有反应性的主要抗原的分子大小为160 kDa、100 kDa、90 kDa和60 kDa，并且对80 kDa、73 kDa和55 kDa的抗原也观察到相对频繁的体液免疫反应。
