Raoult D, Dasch G A
Unité des Rickettsies, Faculté de Médecine, CNRS EP J 0054, Marseille, France.
FEMS Immunol Med Microbiol. 1995 Mar;11(1):13-8. doi: 10.1111/j.1574-695X.1995.tb00073.x.
Sera from patients suffering from Mediterranean spotted fever (i.e. an infection due to Rickettsia conorii) were studied by immunoblot to investigate cross-reactivity. A prevalence of IgM antibodies to Proteus OX 19, Proteus OX 2, to the Rickettsia typhus group, to Legionella pneumophila serovars 4 and 5, to L. bozemanii Wiga and to L. micdadei Tatlock was found. Western blot confirmed that the antibodies were directed against the lipopolysaccharide as demonstrated by proteinase K digestion of the antigens. Cross-adsorptions showed that there is a common cross-reacting epitope among L. bozemanii Wiga, R. typhi and Proteus OX 19 but cross-reacting antibodies to L. micdadei and OX 2 were distinct and independent. This IgM cross-reaction could lead to a misdiagnosis.
对患有地中海斑疹热（即由康氏立克次体感染所致）患者的血清进行免疫印迹研究，以调查交叉反应性。发现存在针对变形杆菌OX19、变形杆菌OX2、斑疹伤寒群立克次体、嗜肺军团菌血清型4和5、博氏军团菌威加菌株以及米氏军团菌塔特洛克菌株的IgM抗体。蛋白质印迹法证实，这些抗体是针对抗原经蛋白酶K消化后所显示的脂多糖。交叉吸附表明，博氏军团菌威加菌株、伤寒立克次体和变形杆菌OX19之间存在共同的交叉反应表位，但针对米氏军团菌和OX2的交叉反应抗体是不同且独立的。这种IgM交叉反应可能导致误诊。
