Gmür Rudolf, Wyss Chris, Xue Yi, Thurnheer Thomas, Guggenheim Bernhard
Institute of Oral Biology, Section of Oral Microbiology and General Immunology, Center for Dental-, Oral Medicine and Maxillofacial Surgery, University of Zürich, Zürich, Switzerland.
Eur J Oral Sci. 2004 Feb;112(1):33-41. doi: 10.1111/j.0909-8836.2004.00103.x.
The objective of this study was to quantitatively compare the bacterial population structure in plaque from the gingival margin of two groups of 21 Chinese patients with gingivitis or necrotizing ulcerative gingivitis (NUG). Subjects were recruited in four dental clinics in Eastern China. Samples were quantitatively assessed by immunofluorescence and fluorescent in situ hybridization for taxa known to be associated with periodontal diseases. The analyses showed that the fusiform taxa (Fusobacterium nucleatum/Fusobacterium periodonticum, Leptotrichia buccalis, Tannerella forsythensis, and Capnocytophaga sp.), Campylobacter rectus, Prevotella intermedia, Prevotella nigrescens, Selenomonas sputigena, and treponemes were present in both groups with high prevalence. Porphyromonas gingivalis and Actinomyces gerencseriae were much more prevalent in the NUG group. Quantitatively, most taxa, including P. gingivalis, F. nucleatum and the treponemes, accounted, on average, for < 3% of the total bacterial cell number. Only P. intermedia/P. nigrescens, P. gingivalis, S. sputigena, A. gerencseriae, and the sum of all monitored suspected periodontal pathogens were significantly increased in the NUG group. The present study demonstrates for both groups a highly diverse plaque composition and suggests that, etiologically, the overall concentration and the concerted effects of the entire group of opportunistic pathogens thriving in NUG-associated plaque are of prime importance.
本研究的目的是对两组共21名患有牙龈炎或坏死性溃疡性牙龈炎（NUG）的中国患者牙龈边缘菌斑中的细菌种群结构进行定量比较。研究对象来自中国东部的四家牙科诊所。通过免疫荧光和荧光原位杂交技术对已知与牙周疾病相关的分类群进行定量评估。分析表明，两组中梭形分类群（具核梭杆菌/牙周梭杆菌、颊纤毛菌、福赛坦氏菌和嗜二氧化碳噬纤维菌属）、直肠弯曲菌、中间普氏菌、变黑普氏菌、生痰月形单胞菌和密螺旋体的患病率都很高。牙龈卟啉单胞菌和杰氏放线菌在NUG组中的患病率更高。从数量上看，大多数分类群，包括牙龈卟啉单胞菌、具核梭杆菌和密螺旋体，平均占细菌细胞总数的不到3%。只有中间普氏菌/变黑普氏菌、牙龈卟啉单胞菌、生痰月形单胞菌、杰氏放线菌以及所有监测的疑似牙周病原体的总和在NUG组中显著增加。本研究表明两组菌斑组成都高度多样化，并提示在病因学上，在与NUG相关的菌斑中大量繁殖的整个机会性病原体组的总体浓度和协同作用至关重要。
