嗜油栖热放线菌SK2中alkB基因的克隆与功能分析。
Cloning and functional analysis of alkB genes in Alcanivorax borkumensis SK2.
作者信息
Hara Akihiro, Baik Sang-ho, Syutsubo Kazuaki, Misawa Norihiko, Smits Theo H M, van Beilen Jan B, Harayama Shigeaki
机构信息
Marine Biotechnology Institute, 3-75-1 Heita, Kamaishi, Iwate 026-0001, Japan. Institute of Biotechnology, ETH-Hönggerberg, HPT D71, CH-8093 Zürich, Switzerland.
出版信息
Environ Microbiol. 2004 Mar;6(3):191-7. doi: 10.1046/j.1462-2920.2003.00550.x.
Alcanivorax is an alkane-degrading marine bacterium which propagates and becomes predominant in crude-oil-containing seawater when nitrogen and phosphorus nutrients are supplemented. To identify the genes responsible for alkane degradation in this organism, two putative genes for alkane hydroxylases were cloned from Alcanivorax borkumensis SK2. They were named alkB1 and alkB2. These genes were subsequently disrupted in A. borkumensis SK2, and the growth phenotypes of the disruptants were examined. The results indicate that the alkB1 gene is responsible for the degradation of short-chain n-alkanes. A double mutant defective in both alkB1 and alkB2 was still able to grow on medium-chain n-alkanes, indicating that genes other than alkB1 and alkB2 are also involved in n-alkane hydroxylation by A. borkumensis SK2.
食烷菌属是一种可降解烷烃的海洋细菌，当添加氮和磷养分时，它会在含原油的海水中繁殖并成为优势菌。为了鉴定该生物体中负责烷烃降解的基因，从博氏食烷菌SK2中克隆了两个假定的烷烃羟化酶基因。它们被命名为alkB1和alkB2。随后在博氏食烷菌SK2中破坏了这些基因，并检测了突变体的生长表型。结果表明，alkB1基因负责短链正构烷烃的降解。alkB1和alkB2均有缺陷的双突变体仍能够在中链正构烷烃上生长，这表明除alkB1和alkB2之外的其他基因也参与了博氏食烷菌SK2的正构烷烃羟化作用。
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