Liu Yongchuang, Xu Siqiong, Li Jialiang, Ouyang Yujie, Gao Shuai, Yang Pan, Chu Cuiwei, He Jian, Yang Tongwen, Ma Keshi, Li Lili, Wang Chao
College of Life Sciences and Agronomy, Zhoukou Normal University, Zhoukou, China.
Key Laboratory of Agricultural Environmental Microbiology, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing, China.
Curr Microbiol. 2025 Jan 4;82(2):69. doi: 10.1007/s00284-024-04053-5.
A bacterial strain P1, capable of degrading diesel and converting thiosulfate to sulfate was isolated from an oil-contaminated soil sample. The cells were Gram-stain-negative, slightly curved rods and motile with a single polar flagellum. Growth of the strain was observed at 4-45 °C (optimum at 28 °C), at pH 4.0-12.0 (optimum at pH 10.0) and with 0-15.0% (w/v) NaCl (optimum at 2.0%). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain P1 was closely related to the members of the genus Limnobacter, with the highest sequence similarity to Limnobacter thiooxidans DSM 13612 (99.8%), followed by Limnobacter alexandrii LZ-4 (99.4%), Limnobacter parvus YS8-69 (98.8%), Limnobacter litoralis KP1-19 (97.6%), and Limnobacter humi UCM-39 (97.5%). The draft genome sequence of strain P1 was 3.40 Mb long, with a DNA G + C content of 52.4%. The average nucleotide identity and digital DNA-DNA hybridization values between strain P1 and the closely related type strains were in the range of 71.8-85.1% and 18.1-28.7%, respectively. The predominant cellular fatty acids of strain P1 included C, summed feature 3 (C ɷ7c and/or C ɷ6c), summed feature 8 (C ɷ7c and/or C ɷ6c), and summed feature 7 (C ɷ6c and/or C ɷ7c and/or C cyclo). In addition, the main polar lipid was composed of diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol. Q-8 was the sole respiratory quinone. Based on the polyphasic characterization, strain P1 (= KCTC 72814 = CCTCC AB 2019403) represents a novel species of the genus Limnobacter, for which the name Limnobacter olei sp. nov. is proposed.
从一个受石油污染的土壤样本中分离出了一株能够降解柴油并将硫代硫酸盐转化为硫酸盐的细菌菌株P1。该菌株细胞革兰氏染色阴性，呈微弯杆状，具单根极生鞭毛，能运动。该菌株在4-45℃（最适温度28℃）、pH 4.0-12.0（最适pH 10.0）以及0-15.0%（w/v）NaCl（最适浓度2.0%）条件下均可生长。基于16S rRNA基因序列的系统发育分析表明，菌株P1与Limnobacter属的成员密切相关，与硫氧化Limnobacter thiooxidans DSM 13612的序列相似性最高（99.8%），其次是亚历山大Limnobacter alexandrii LZ-4（99.4%）、微小Limnobacter parvus YS8-69（98.8%）、滨岸Limnobacter litoralis KP1-19（97.6%）和湿地Limnobacter humi UCM-39（97.5%）。菌株P1的基因组草图序列长度为3.40 Mb，DNA G+C含量为52.4%。菌株P1与密切相关的模式菌株之间的平均核苷酸同一性和数字DNA-DNA杂交值分别在71.8-85.1%和18.1-28.7%范围内。菌株P1的主要细胞脂肪酸包括C、总和特征3（C ɷ7c和/或C ɷ6c）、总和特征8（C ɷ7c和/或C ɷ6c）以及总和特征7（C ɷ6c和/或C ɷ7c和/或C环）。此外，主要极性脂由双磷脂酰甘油、磷脂酰乙醇胺和磷脂酰甘油组成。Q-8是唯一的呼吸醌。基于多相分类特征，菌株P1（=KCTC 72814=CCTCC AB 2019403）代表Limnobacter属的一个新物种，为此提出名为油Limnobacter olei sp. nov. 的名称。
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