Shubair M, Stanek R, White S, Larsen B
Department of Microbiology, Marshall University School of Medicine, Huntington, W. Va. 25701.
Gynecol Obstet Invest. 1992;34(4):229-33. doi: 10.1159/000292767.
The effects of a 0.5% aqueous chlorhexidine gluconate (CHG) douche on the normal vaginal flora of twenty healthy nonpregnant volunteers were investigated. The douche was applied in a premeasured 180-ml quantity daily for 7 consecutive days. Specimens for aerobic and anaerobic culture of the vaginal flora were obtained on 3 occasions from each volunteer, once before and twice after CHG use. The culture results were analyzed qualitatively and quantitatively. A blood sample was also collected within 24 h of the last CHG application to determine serum CHG concentration. Lactobacillus spp., Gardnerella vaginalis and Staphylococcus epidermidis were the most prevalent aerobic bacteria at all phases of the study and Bacteroides spp. were the most prevalent anaerobes. The composition of the normal flora was essentially the same 30 days after the last dose as the pretreatment flora. Small amounts of CHG were detected in the serum of all but one subject. No untoward effects on the participants were observed. Candida albicans counts were slightly higher, but prevalence was not significantly increased at the end of the study.
研究了0.5%葡萄糖酸氯己定（CHG）水溶液灌洗对20名健康非妊娠志愿者正常阴道菌群的影响。每天以预先测量的180毫升量进行灌洗，连续7天。从每位志愿者身上分3次采集阴道菌群需氧和厌氧培养的标本，一次在使用CHG之前，两次在使用CHG之后。对培养结果进行定性和定量分析。在最后一次使用CHG后24小时内还采集了血样，以测定血清CHG浓度。在研究的所有阶段，乳酸杆菌属、阴道加德纳菌和表皮葡萄球菌是最常见的需氧菌，拟杆菌属是最常见的厌氧菌。最后一剂后30天正常菌群的组成与治疗前基本相同。除一名受试者外，所有受试者的血清中均检测到少量CHG。未观察到对参与者有不良影响。白色念珠菌计数略有升高，但在研究结束时其患病率没有显著增加。
