Patton D L, Sweeney Y C, Rabe L K, Hillier S L
Department of Obstetrics and Gynecology, University of Washington, Seattle 98195-6460, USA.
Sex Transm Dis. 1996 Nov-Dec;23(6):489-93. doi: 10.1097/00007435-199611000-00009.
To characterize normal vaginal microflora of pig-tailed macaques and to evaluate two commonly used intravaginal compounds, chlorhexidine (CHG), a vaginal antiseptic (Surgilube, E. Fougera, Melville, NY), and benzalkonium chloride (BZK) (spermicidal contraceptive) in this monkey model to assess effects on the vaginal microflora.
Vaginal swabs were collected for microbiologic analysis to characterize normal flora. Subsequently, the vagina was exposed to either CHG or BZK twice at 24-hour intervals.
The vaginal microflora of 26 pig-tailed macaques was found to be remarkably similar to the vaginal flora of the human with respect to frequency of vaginal colonization by H2O2-producing lactobacilli, Prevotella species, and several other microorganisms. After two vaginal applications at 24-hour intervals, CHG had only small effects on the vaginal microflora of five animals. By contrast, BZK applied by the same protocol had profound adverse effects on the lactobacilli and Peptostreptococcus and more transient effects on vaginal Prevotella and viridans streptococci of six animals.
These observations demonstrate that the vaginal microflora of the pig-tailed macaque is a useful model in which to further evaluate newly developed intravaginal contraceptives that may be microbicidal and/or virucidal before widespread intravaginal use in women.
描述豚尾猕猴的正常阴道微生物群，并在该猴模型中评估两种常用的阴道内化合物，即阴道消毒剂氯己定（CHG）（舒洁宁，E. Fougera公司，纽约梅尔维尔）和苯扎氯铵（BZK）（杀精子避孕药），以评估它们对阴道微生物群的影响。
采集阴道拭子进行微生物分析以描述正常菌群。随后，阴道在24小时间隔内接受CHG或BZK处理两次。
发现26只豚尾猕猴的阴道微生物群在产生过氧化氢的乳酸杆菌、普雷沃菌属和其他几种微生物在阴道定植的频率方面与人的阴道菌群非常相似。在24小时间隔内进行两次阴道给药后，CHG对五只动物的阴道微生物群只有轻微影响。相比之下，按照相同方案应用的BZK对六只动物的乳酸杆菌和消化链球菌有严重的不良影响，对阴道普雷沃菌属和草绿色链球菌有更短暂的影响。
这些观察结果表明，豚尾猕猴的阴道微生物群是一个有用的模型，可用于在新开发的可能具有杀菌和/或杀病毒作用的阴道避孕药在女性中广泛阴道使用之前进一步评估它们。
