Serrano-Carreon L, Hathout Y, Bensoussan M, Belin J M
ENS.BANA, Laboratoire de Biotechnologie, Université de Bourgogne, Dijon, France.
J Chromatogr. 1992 Dec 11;584(1):129-33. doi: 10.1016/0378-4347(92)80018-l.
Bond Elut aminopropyl columns were used to purify the different lipid classes of Trichoderma harzianum and Trichoderma viride. This methodology permitted good separation of the fungal lipid classes in less time than traditional techniques. The incorporation of [1 (14)C]linoleic acid into neutral lipids, free fatty acids and phospholipids was quantified for both strains. The fatty acid profile of the different lipid classes of these fungal strains was determined as a function of culture time.
使用Bond Elut氨丙基柱来纯化哈茨木霉和绿色木霉的不同脂质类别。这种方法比传统技术能在更短时间内实现对真菌脂质类别的良好分离。对这两种菌株，均对[1 (14)C]亚油酸掺入中性脂质、游离脂肪酸和磷脂的情况进行了定量。测定了这些真菌菌株不同脂质类别的脂肪酸谱随培养时间的变化情况。
