Prasad M R, Jones R M, Young H S, Kaplinsky L B, Das D K
Department of Surgery, University of Connecticut School of Medicine, Farmington 06032.
J Chromatogr. 1988 Jul 15;428(2):221-8. doi: 10.1016/s0378-4347(00)83912-2.
Gas chromatographic analysis revealed that polyunsaturated fatty acids such as arachidonic acid and total tissue free fatty acids isolated from an aminopropyl bonded-phase column yield a two- to three-fold higher recovery of arachidonic acid as compared to those isolated from thin-layer chromatographic plates. This method was further improved by packing the aminopropyl bonded phase in glass columns, since the glass column significantly eliminated the other contaminants (from polypropylene columns) coeluting with fatty acids in both a neutral lipid thin-layer chromatographic system and on a 5% DEGS-PS column of gas chromatographic analysis. In aminopropyl bonded-phase columns, the standard triglycerides and phospholipids were completely separated from free fatty acids as judged by gas chromatographic analysis. These results warrant the use of an aminopropyl bonded-phase column for the isolation of free fatty acids to obtain better recovery of polyunsaturated fatty acids.
气相色谱分析表明，从氨基丙基键合相柱分离得到的花生四烯酸等多不饱和脂肪酸和总组织游离脂肪酸，与从薄层层析板分离得到的相比，花生四烯酸的回收率高出两到三倍。通过将氨基丙基键合相填充到玻璃柱中，该方法得到了进一步改进，因为在中性脂质薄层层析系统和气相色谱分析的5% DEGS - PS柱上，玻璃柱显著消除了与脂肪酸共洗脱的其他污染物（来自聚丙烯柱）。通过气相色谱分析判断，在氨基丙基键合相柱中，标准甘油三酯和磷脂与游离脂肪酸完全分离。这些结果证明使用氨基丙基键合相柱分离游离脂肪酸以获得更好的多不饱和脂肪酸回收率是合理的。
