Fukudome H, Tanaka K
Department of Anatomy, Faculty of Medicine, Tottori University, Yonago, Japan.
J Electron Microsc (Tokyo). 1992 Oct;41(5):357-63.
Perfusion with a dilute saponin solution before perfusion and immersion fixation with 0.5% glutaraldehyde and 0.5% formaldehyde, followed by routine processing for high-resolution scanning electron microscopy, allows a variety of cytoskeletal elements to be well preserved. Membranous organelles are also clearly seen, allowing the three-dimensional relationships of membranes and filaments within the cell to be directly observed. In particular, in the rat intermediate filaments were seen to terminate on smooth endoplasmic reticulum and nuclear envelopes of intestinal epithelia, and to form networks between mitochondria and rough endoplasmic reticulum in podocytes of renal glomeruli.
