Henriksson A, Conway P L
Department of General and Marine Microbiology, University of Göteborg, Sweden.
J Gen Microbiol. 1992 Dec;138(12):2657-61. doi: 10.1099/00221287-138-12-2657.
The mechanism by which Lactobacillus fermentum strain 104-S adheres to porcine squamous epithelium was investigated by studying the adsorption to epithelial cells, and control surfaces, of radioactively labelled material released from the bacterial cells by water extraction. The released material was fractionated by gel filtration and the adsorption of pronase-sensitive and -resistant material in the various fractions to porcine gastric tissue and the control surfaces of polystyrene and immobilized bovine serum albumin (BSA) was determined. The fraction with affinity for the epithelium was characterized by enzymic degradation, periodate oxidation, lipid extraction, and protein and carbohydrate analyses. The adsorption pattern of radioactively labelled crude released material mimicked the adhesion of whole labelled cells to polystyrene and to gastric squamous tissue pieces. On fractionation, the pattern of adsorption to polystyrene and BSA was different from that obtained for the tissue pieces. Considerably less labelled pronase-stable material bound to surfaces of polystyrene and BSA, as compared with the tissue, suggesting that the pronase-resistant component has a tissue-specific affinity. After pronase treatment of the fraction of M(r) about 20,000 (20 K) containing labelled components with affinity for the epithelium, only saccharides were detected. Radioactivity was lost after hydrolysis with HCl, and therefore this pronase-resistant labelled component must be a saccharide. It is concluded that protein moieties in the extract have an affinity for several surfaces, including polystyrene, and that saccharide moieties have a specific affinity for the gastric squamous epithelium.
通过研究水提取法从发酵乳杆菌104 - S菌株释放的放射性标记物质对上皮细胞和对照表面的吸附情况，来探究该菌株对猪鳞状上皮的黏附机制。将释放的物质通过凝胶过滤进行分级分离，并测定各分级中对链霉蛋白酶敏感和抗性的物质对猪胃组织以及聚苯乙烯和固定化牛血清白蛋白（BSA）对照表面的吸附情况。对具有上皮细胞亲和力的分级进行酶解、高碘酸盐氧化、脂质提取以及蛋白质和碳水化合物分析，以此来对其进行表征。放射性标记的粗释放物质的吸附模式与整个标记细胞对聚苯乙烯和胃鳞状组织块的黏附情况相似。分级分离后，对聚苯乙烯和BSA的吸附模式与对组织块的吸附模式不同。与组织相比，与聚苯乙烯和BSA表面结合的标记链霉蛋白酶稳定物质要少得多，这表明链霉蛋白酶抗性成分具有组织特异性亲和力。用链霉蛋白酶处理含有对上皮细胞有亲和力的标记成分、相对分子质量约为20000（20K）的分级后，仅检测到糖类。用盐酸水解后放射性消失，因此这种链霉蛋白酶抗性标记成分必定是一种糖类。得出的结论是，提取物中的蛋白质部分对包括聚苯乙烯在内的多个表面具有亲和力，而糖类部分对胃鳞状上皮具有特异性亲和力。
