Greene J D, Klaenhammer T R
Department of Food Science, North Carolina State University, Raleigh 27695-7624.
Appl Environ Microbiol. 1994 Dec;60(12):4487-94. doi: 10.1128/aem.60.12.4487-4494.1994.
A quantitative assay performed with bacterial cells labelled with [3H]thymidine was used to investigate factors involved in the adherence of human isolates Lactobacillus acidophilus BG2FO4 and NCFM/N2 and Lactobacillus gasseri ADH to human Caco-2 intestinal cells. For all three strains, adherence was concentration dependent, greater at acidic pH values, and significantly greater than adherence of a control dairy isolate, Lactobacillus delbrueckii subsp. bulgaricus 1489. Adherence of L. acidophilus BG2FO4 and NCFM/N2 was decreased by protease treatment of the bacterial cells, whereas adherence of L. gasseri ADH either was not affected or was enhanced by protease treatment. Putative surface layer proteins were identified on L. acidophilus BG2FO4 and NCFM/N2 cells but were not involved in adherence. Periodate oxidation of bacterial cell surface carbohydrates significantly reduced adherence of L. gasseri ADH, moderately reduced adherence of L. acidophilus BG2FO4, and had no effect on adherence of L. acidophilus NCFM/N2. These results indicate that Lactobacillus species adhere to human intestinal cells via mechanisms which involve different combinations of carbohydrate and protein factors on the bacterial cell surface. The involvement of a secreted bridging protein, which has been proposed as the primary mediator of adherence of L. acidophilus BG2FO4 in spent culture supernatant (M.-H. Coconnier, T. R. Klaenhammer, S. Kernéis, M.-F. Bernet, and A. L. Servin, Appl. Environ. Microbiol. 58:2034-2039, 1992), was not confirmed in this study. Rather, a pH effect on Caco-2 cells contributed significantly to the adherence of this strain in spent culture supernatant.(ABSTRACT TRUNCATED AT 250 WORDS)
采用对用[3H]胸苷标记的细菌细胞进行的定量分析，来研究嗜酸乳杆菌BG2FO4和NCFM/N2以及加氏乳杆菌ADH这些人源分离株与人类Caco-2肠细胞黏附过程中的相关因素。对于这三株菌，黏附均呈浓度依赖性，在酸性pH值下更高，且显著高于对照乳源分离株德氏乳杆菌保加利亚亚种1489的黏附。嗜酸乳杆菌BG2FO4和NCFM/N2的细菌细胞经蛋白酶处理后黏附性降低，而加氏乳杆菌ADH的黏附性要么不受影响，要么经蛋白酶处理后增强。在嗜酸乳杆菌BG2FO4和NCFM/N2细胞上鉴定出了假定的表层蛋白，但它们不参与黏附。细菌细胞表面碳水化合物的高碘酸盐氧化显著降低了加氏乳杆菌ADH的黏附，适度降低了嗜酸乳杆菌BG२FO4的黏附，而对嗜酸乳杆菌NCFM/N2的黏附没有影响。这些结果表明，乳杆菌属通过涉及细菌细胞表面碳水化合物和蛋白质因子不同组合的机制黏附于人类肠细胞。曾有人提出嗜酸乳杆菌BG2FO4在培养上清液中的黏附主要介导因子是一种分泌型桥接蛋白（M.-H. 科科尼尔、T.R. 克莱恩哈默、S. 克尔内斯、M.-F. 贝内、A.L. 塞尔万，《应用与环境微生物学》58:2034 - 2039, 1992），但本研究未证实这一点。相反，pH对Caco-2细胞的影响对该菌株在培养上清液中的黏附起了显著作用。（摘要截短至250词）
