Gusils C, Oppezzo O, Pizarro R, González S
Centro de Referencias para Lactobaciolos-Consejo Nacional de Investigaciones Científicas y Tecnológicas, Tucumán, Argentina.
Can J Microbiol. 2003 Jul;49(7):472-8. doi: 10.1139/w03-055.
In the present work, interactions between three Lactobacillus strains (Lactobacillus fermentum CRL1015, Lactobacillus animalis CRL1014, and Lactobacillus fermentum CRL1016) and chicken small intestinal mucus were determined. Three lactobacilli isolated from chicken and selected by their potentially probiotic properties were able to grow in mucus preparations. Three peaks from gel filtration chromatography of intestinal mucus were obtained. The adhesion to three mucus fractions (I, II, and III), especially fraction III, was higher (P < 0.01) in L. fermentum CRL1015 than L. animalis CRL1014. Pretreatment of this fraction with proteases and metaperiodate showed lower (P < 0.01) adhesion values than that of the control, suggesting that a glycoprotein from the mucus acts as a receptor for L. fermentum CRL1015. Highest adhesion values were obtained at pH 7 and 42 degrees C, and neither the removal of divalent cations with ethylenediaminetetraacetic acid (EDTA) nor the addition of calcium produced significant variation from the adhesion values of the control (P > 0.01). This adhesion was only inhibited by N-acetyl-glucosamine. Salmonella pullorum and Salmonella gallinarum showed high (P < 0.01) values of adhesion to chick intestinal mucus. The results obtained from assays of the inhibition of adherence of Salmonella spp. to mucus, immobilized in polystyrene tissue culture wells, indicated that the pathogen adhesion was not reduced by lactobacilli (P > 0.05) or their spent culture supernatants (P > 0.05), suggesting that these strains did not interfere with the binding sites for Salmonella spp. adhesion to the small intestinal mucus.
在本研究中，测定了三种乳酸杆菌菌株（发酵乳杆菌CRL1015、动物乳杆菌CRL1014和发酵乳杆菌CRL1016）与鸡小肠黏液之间的相互作用。从鸡体内分离出的、根据其潜在益生菌特性挑选出的三种乳酸杆菌能够在黏液制剂中生长。从小肠黏液的凝胶过滤色谱中获得了三个峰。发酵乳杆菌CRL1015对三种黏液组分（I、II和III），尤其是组分III的黏附力高于（P<0.01）动物乳杆菌CRL1014。用蛋白酶和高碘酸盐对该组分进行预处理后，其黏附值低于（P<0.01）对照组，这表明黏液中的一种糖蛋白充当了发酵乳杆菌CRL1015的受体。在pH 7和42℃时获得了最高黏附值，用乙二胺四乙酸（EDTA）去除二价阳离子或添加钙均未使黏附值与对照组产生显著差异（P>0.01）。这种黏附仅被N-乙酰葡糖胺抑制。鸡白痢沙门氏菌和鸡伤寒沙门氏菌对鸡小肠黏液的黏附值较高（P<0.01）。对沙门氏菌属黏附于固定在聚苯乙烯组织培养孔中的黏液的抑制试验结果表明，乳酸杆菌（P>0.05）或其用过的培养上清液（P>0.05）不会降低病原体的黏附，这表明这些菌株不会干扰沙门氏菌属黏附于小肠黏液的结合位点。
